WOAH hosts the second PROVNA2 webinar on Entomology – Use of Mosquito traps

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Photo (c) Sara Seneca

The objective of the meeting was to show how to set up and use the mosquito traps provided in the framework of the Project (for the beneficiary countries that requested them) and to discuss the preparation of insect samples to be stored until they are identified.

The session was led by Silvio G. D’Alessio (Entomology Unit – IZS Teramo), who discussed different types of traps, their components, and proper assembly procedures, including technical details about power sources and attractants. The session concluded with guidance on sample handling and storage, followed by announcements about upcoming training sessions and distribution of traps to participating countries.

BG Pro (Biogents AG) standing configuration (Sentinel style). Picture (c) Silvio G. D’Alessio (IZS) 2025

Key Focus: Effective Mosquito Collection

The training explored strategies for collecting adult mosquitoes in diverse environments by leveraging mosquito ecology, behavior, and environmental factors. Attendees learned that attractants such as CO₂ (from dry ice, gas, or fermentation), light, and chemical lures can target specific mosquito species, especially nocturnal and human-biting types.

BG Pro (Biogents AG) hanging configuration (CDC style). Picture (c) Silvio G. D’Alessio (IZS) 2025.

Understanding Trap Technologies

The webinar compared the technical specifications of common mosquito traps and focused on the functioning of those purchased in the framework of the project: the BG Pro (Biogents AG). These traps can operate in two configurations: hanging (CDC style) and standing (Sentinel style)—and can be powered via battery, main supply, or a compact power bank. These features make it suitable for various field conditions and extended use.

Species identification under a stereomicroscope. Picture (c) Silvio G. D’Alessio (IZS) 2025.

Field Deployment and Sample Management

Silvio G. D’Alessio also provided an overview on trap assembly, selection of attractants, and site criteria (e.g., accessible, shaded, discreet). Once deployed, careful handling of the collection bags ensures samples are preserved without damage or contamination. The training emphasized some key steps from the field to the laboratory of adult mosquitos samples:

  • Killing and cooling collected mosquitoes
  • Transfer the insects into a falcon tube
  • Species identification under a stereomicroscope
  • Pooling female mosquitoes by species in labelled tubes
  • Storing samples at -20°C or -80°C for virological analysis, avoiding thawing (defrost) and re freezing

Tools and Resources Provided in the framework of PROVNA2

Each requesting country received a standardized kit including BG Pro traps, attractants, collection bags, and power cables.

Additionally, participants were given access to multilingual materials:

  • BG Pro user manual
  • Protocols in English, French, and Arabic on:
          • Collection of vectors – use of traps
          • Animal sampling protocols
          • Accessory specifications of traps
          • Definition of suspected and confirmed RVF cases
  • Instructional videos in English and French on how to set-up the traps 

Looking Ahead

This webinar marks an important step in building regional capacity for arbovirus surveillance, particularly for diseases like Rift Valley Fever. By standardizing entomological practices and equipment use, PROVNA 2 strengthens early warning systems and supports coordinated response strategies in the North African region.

Presentations

D'Alessio
D'Alessio

PDF - 7.22MB

Acknowledgements

This project is funded by USDA APHIS and benefits from the technical support of IZS Teramo.

Previous PROVNA Events

WOAH hosts first PROVNA 2 webinar on the use of Satellite Data

March 18, 2025
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WOAH hosts a workshop on PROVNA project and Foresight, in the framework of REMESA.

July 31, 2024
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Launch of PROVNA 2: Establishment of a Risk-Based Surveillance System for Mosquito-Borne Diseases in North Africa

November 12, 2024
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